DOI:

10.37988/1811-153X_2020_2_73

Markers of osteoblasts and osteoclasts in patients with dental implants

Downloads

Authors

  • T.G. Makhmudov 1, PhD in Medical Sciences, assistant professor of the Prosthetic dentistry Department
  • 1 Azerbaijan Medical University, AZ1012, Baku, Azerbaijan

Abstract

It has been microscopically established that bone remodeling consists in bone resorption by osteoclasts with subsequent bone formation by osteoblasts. One of the typical markers of osteoblasts is alkaline phosphatase (ALP), tartrate resistant acidic phosphatase (TRAP) is a marker of osteoclastic activity and bone resorption. In the literature, information about the study of these markers in the oral fluid (OF) in patients with dental implants is limited. Objectives: determination of the activity of ALP and TRAP in pancreatic cancer in patients with dental implants. Materials and methods. Dental intraosseous implantation was performed in 164 patients, the average age was 54.6±4.2 years. 641 screw intraosseous implants of the MIS system were installed. The indices Green—Vermillion and Muhleman—Cowell were determined. The activity of ALP was measured using a set of chemical reagents alkaline phosphatase — UTS by the hydrolysis of phosphoric acid ester on an automatic biochemical analyzer. TRAP5b activity was determined by ELISA using a test kit. Studies were carried out before implant placement, after 7, 14, 21 days, after 3 and 6 months. Results. Immediately prior to dental implantation, the average level of the OHI-S and Mulleman—Cowell index was 0.62±0.06 and 0.43±0.04. Patients were divided into 2 groups: group I —119 patients without complications, group II — 45 patients with acute mucositis. In comparison with the control, a statistically insignificant increase in the activity of ALP was revealed before dental implantation; later, in the presence of implants, depending on the complication that occurred, it was multidirectional. An increase in TRAP activity was observed in the first 14 days in both groups, especially pronounced in group II. Conclusions. An increase in ALP activity is not statistically significant. The activity of TRAP in OF in patients with complicated and uncomplicated course after the implantation period was increased during 6 months, and the maximum activity was noted after 14 days, exceeding the initial one by 3.0 times (p<0.01). The determination of the activity of TRAP in the OF after implantation can be used as biochemical indicator of osteoclast function.

Key words:

dental implant, oral fluid, alkaline phosphatase, tartrate acid resistant phosphatase, osseointegration

For Citation

[1]
Makhmudov T.G. Markers of osteoblasts and osteoclasts in patients with dental implants. Clinical Dentistry (Russia).  2020; 2 (94): 73—77. DOI: 10.37988/1811-153X_2020_2_73

References

  1. Meng H.-W., Chien E.Y., Chien H.-H. Dental implant bioactive surface modifications and their effects on osseointegration: A review. — Biomark Res. — 2016; 4: 24. PMID: 27999672
  2. Müller H.-D., Caballé-Serrano J., Lussi A., Gruber R. Inhibitory effect of saliva on osteoclastogenesis in vitro requires toll-like receptor 4 signaling. — Clin Oral Investig. — 2017; 21 (8): 2445—52. PMID: 28101679
  3. Ballantyne E. Bisphosphonates: Possible modes of action and implications for dental implant treatment. A Review of the literature. — Journal of General Practice. — 2015; 3: 1. DOI: 10.4172/2329-9126.1000192
  4. Soares P.B.F., Moura C.C.G., Rocha Junior H.A., Dechichi P., Zanetta-Barbosa D. Biological characterization of implant surfaces — in vitro study. — Revista de Odontologia da UNESP. — 2015; 44 (4): 195—9. DOI: 10.1590/1807-2577.1087
  5. Tonelli P., Duvina M., Barbato L., Biondi E., Nuti N., Brancato L., Rose G.D. Bone regeneration in dentistry. — Clin Cases Miner Bone Metab. — 2011; 8 (3): 24—8. PMID: 22461825
  6. AlMoharib H.S., AlMubarak A., AlRowis R., Geevarghese A., Preethanath R.S., Anil S. Oral fluid based biomarkers in periodontal disease: Part 1. Saliva. — J Int Oral Health. — 2014; 6 (4): 95—103. PMID: 25214743
  7. Gelnin E.V. Dynamics of acid and alkaline phosphatases in the oral fluid at outpatient surgery of odontogenic inflammatory jaw diseases and shortness of teething wisdom. — Advances in current natural sciences. — 2015; 1—4: 561—4 (In Russ.).
  8. Jazaeri M., Malekzadeh H., Abdolsamadi H., Rezaei-Soufi L., Samami M. Relationship between salivary alkaline phosphatase enzyme activity and the concentrations of salivary calcium and phosphate ions. — Cell J. — 2015; 17 (1): 159—62. PMID: 25870846
  9. Pohodenko-Chudakova I.O., Karsuk Yu.V. Changes of biochemical indices of oral fluid in operation of the prosthetic with support on dental implants. — Stomatologist (Belorussia). — 2019; 1 (32): 38—43 (In Russ.).
  10. Lysov D.N., Zarubina E.G. Clinical and laboratory characteristics of bone homeostasis after uncomplicated installation of dental implants. — Bulletin of Medical Institute “REAVIZ”: Rehabilitation, Physician and Health. — 2018; 4 (34): 106—12 (In Russ.).
  11. Pohoden’ko-Chudakova I.O., Karsjuk Ju.V. Justification of the study on the development of a system for predicting the outcomes of dental implantation. Analytical review of the literature. — Bulletin of Vitebsk State Medical University. — 2014; 1: 6—12 (In Russ.).
  12. Haga M., Fujii N., Nozawa-Inoue K., Nomura S., Oda K., Uoshima K., Maeda T. Detailed process of bone remodeling after achievement of osseointegration in a rat implantation model. — Anat Rec (Hoboken). — 2009; 292 (1): 38—47. PMID: 18727113
  13. Nassrawin N.A. Detection of ostecalcin in gingival crevicular fluid in a group of orthodontic patients. — J Int Soc Prev Community Dent. — 2018; 8 (2): 168—173. PMID: 29780743
  14. Goto T. Osseointegration and Dental Implants. — Clin Calcium. — 2014; 24 (2): 265—71 (In Japan.). PMID: 24473360
  15. Mello A.S.S., Santos P.L., Marquesi A., Queiroz T.P., Margonar R., Faloni A.P.S. Some aspects of bone remodeling around dental implants. — Revista Clínica de Periodoncia, Implantología y Rehabilitación Oral. — 2018; 11 (1): 49—53. DOI: 10.1016/j.piro.2015.12.001

Downloads

Published on

July 25, 2020