DOI:

10.37988/1811-153X_2024_3_6

Antiadhesive and antimicrobial effect of titanium oxide coating with anatase structure in in vitro experiments for medical implants

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Authors

  • V.N. Tsarev 1, Doctor of Science in Medicine, full professor of the Microbiology, virology, immunology department, director of the Medico-dental research Institute
    ORCID: 0000-0002-3311-0367
  • M.S. Podporin 1, PhD in Medical Sciences, researcher at the Molecular biology research Laboratory
    ORCID: 0000-0001-6785-0016
  • T.V. Tsareva 1, PhD in Medical Sciences, associate professor of the Microbiology, virology, immunology Department
    ORCID: 0000-0001-9571-0520
  • V.V. Tsareva 1, researcher at the Laboratory of experimental and clinical ophthalmology
  • M.G. Kozodaev 2, Ph.D in Physical and Mathematical Sciences, senior researcher at the Center of shared facilities in nanotechnology
    ORCID: 0000-0003-2318-6832
  • E.V. Ippolitov 1, Doctor of Science in Medicine, professor, leading researcher of the Molecular biology research Laboratory
    ORCID: 0000-0003-1737-0887
  • 1 Russian University of Medicine, 127006, Moscow, Russia
  • 2 MIPT, 141701, Dolgoprudny, Russia

Abstract

The combination of strength characteristics of titanium alloys, in particular Ti6Al4V, as well as the pronounced bioactive properties of titanium oxide coatings are of significant interest for their joint use in medical structures, which makes necessary to examine their anti-adhesive and antimicrobial properties. This problem takes priority in prosthetics of joints (hip, knee), complex prosthetics in the maxillofacial region (for gunshot and shrapnel wounds), as well as in orthopedic correction after head and neck surgery. Purpose of the study — determination of adhesion of microorganism strains to samples of titanium alloy Ti6Al4V ELI (ASTM F136 standard) with various types of the surface treatment, including titanium oxide coating with an anatase crystalline structure obtained via atomic layer deposition.
Materials and methods.
Properties of titanium alloy Ti6Al4V samples with various types of surface treatment, including the growth of a TiO2 coating with an anatase crystalline structure (samples prepared by CONMET LLC, Moscow), were studied, which are considered as likely candidates for complex orthopedic treatment of patients of various profiles (in particular, using dental implants and artificial joints). Microbiological and molecular biological methods were used, which make it possible to quantify the indicators of microbial adhesion in vitro to the test samples.
Results.
For the first time, the results of a comparative assessment of adhesion degree of sanitary-important microorganisms (Staphylococcus aureus, Escherichia coli, Candida albicans) and anaerobic bacteria (Actinomyces israelii, Porphyromonas gingivalis, Fusobacterium necroforum) to samples made of Ti6Al4V alloy with titanium oxide anatase coating have been obtained.
Conclusion.
The lowest adhesion values for all tested strains of microorganisms, including periodontal pathogenic species of anaerobic bacteria and yeast fungi of the genus Candida, were detected when using Ti6Al4V alloy samples covered with a TiO2 anatase coating, which can be interpreted as the bactericidal and fungicidal properties of this coating.

Key words:

titanium oxide, crystalline structure, anatase, microbial adhesion, ESKAPE strains, anaerobic species, yeast fungi

For Citation

[1]
Tsarev V.N., Podporin M.S., Tsareva T.V., Tsareva V.V., Kozodaev M.G., Ippolitov E.V. Antiadhesive and antimicrobial effect of titanium oxide coating with anatase structure in in vitro experiments for medical implants. Clinical Dentistry (Russia).  2024; 27 (3): 6—13. DOI: 10.37988/1811-153X_2024_3_6

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Received

July 12, 2024

Accepted

August 20, 2024

Published on

October 2, 2024