DOI:

10.37988/1811-153X_2024_2_66

Effect of recombinant lactoferrin on the antimicrobial activity of phytocomposition Mucosept against S. intermedius and C. albicans

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Authors

  • Yu.S. Kiparisov 1, PhD in Medical Sciences, assistant professor of the Prosthodontics Department
    ORCID: 0000-0001-6046-3777
  • D.G. Kiparisova 1, postgraduate at the Prosthodontics and orthodontics Department
    ORCID: 0000-0003-1805-3365
  • A.P. Varukha 2, assistant at the Dental care organization, management, and prevention of dental diseases Department
    ORCID: 0000-0002-7676-1557
  • M.S. Podporin 3, PhD in Medical Sciences, researcher at the Molecular biology research Laboratory of the Medico-dental research Institute
    ORCID: 0000-0001-6785-0016
  • V.N. Tsarev 3, PhD in Medical Sciences, professor, director of the Medico-dental research Institute
    ORCID: 0000-0002-3311-0367
  • E.V. Ippolitov 3, PhD in Medical Sciences, professor, leading researcher of the Molecular biology research Laboratory
    ORCID: 0000-0003-1737-0887
  • V.V. Podporina 3, 5th year student at the Medical Faculty
    ORCID: 0000-0001-6065-1997
  • S.D. Arutyunov 3, PhD in Medical Sciences, full professor of the Digital dentistry Department
    ORCID: 0000-0001-6512-8724
  • 1 South Ural State Medical University, 454092, Chelyabinsk, Russia
  • 2 Stavropol State Medical University, 355017, Stavropol, Russia
  • 3 Russian University of Medicine, 127006, Moscow, Russia

Abstract

The peculiarities of changes in the antimicrobial action of phytopreparations in complex with the protein lactoferrin with respect to microbial cultures were studied. The hypothesis about the possibility of prophylactic application of this antimicrobial complex against pathogens initiating the development of infectious diseases of the oral cavity was tested.
Materials and methods.
 Pre-prepared bacterial suspension was inoculated into nutrient broth with or without preliminary addition of lactoferrin, and the tested samples of phytocompositions were added. The optical density of the microbial suspension was measured during automatic cultivation in an RTS-8 bioreactor (BioSan, Latvia). Culture development curves plotted against these data were analyzed.
Results.
In the dynamics of S. intermedius and C. albicans development, statistically significantly significant differences of maximum optical density were observed when adding Mucosept versus Tonzinal, both with lactoferrin (for S. intermedius p=0.0088, for C. albicans p=0.0090) and without its addition (for S. intermedius p=0.0086, for C. albicans p=0.0283). Addition of lactoferrin to the nutrient medium composition promotes the delay of exponential cell development for an average of 6 hours. When using Mucosept, lower values of optical density indices at key points of development were observed when analyzing the cultivation of bacterial (average decrease by 13%) and fungal populations (average decrease by 23%) depending on the preliminary addition of lactoferrin to the nutrient medium.
Conclusions.
The possible use of phytocompositions with lactoferrin-containing preparations in complex periodontal treatment, including patients with oncological profile, can contribute to the normalization of microbiotic indices. The RTS-8 bioreactor allows obtaining reproducible results, is available and can be recommended for obtaining objective, comparable, reliable data on antimicrobial properties of lactoferrin.

Key words:

mucosept, lactoferrin, mucositis, oncology, bioreactor, S. intermedius, C. albicans

For Citation

[1]
Kiparisov Yu.S., Kiparisova D.G., Varukha A.P., Podporin M.S., Tsarev V.N., Ippolitov E.V., Podporina V.V., Arutyunov S.D. Effect of recombinant lactoferrin on the antimicrobial activity of phytocomposition Mucosept against S. intermedius and C. albicans. Clinical Dentistry (Russia).  2024; 27 (2): 66—75. DOI: 10.37988/1811-153X_2024_2_66

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Received

January 20, 2024

Accepted

June 16, 2024

Published on

June 28, 2024