Comorbidity of periodontitis and herpes in the postcovid period

Abstract

Improving the diagnosis and provision of dental care for patients with chronic periodontitis in combination with herpetic diseases is an urgent goal of many studies. At the same time, the COVID-19 pandemic, including the course of its post-covid period, has somewhat changed the view of specialists on the diagnosis and treatment of these comorbid diseases. The aim of the study was a metagenomic analysis of the microbiota of patients with periodontitis in combination with herpetic lesions in the postcovid period. >. 28 people aged 18—19 years were examined: 8 patients with chronic periodontitis and herpes of the lips in the postcovid period (group I), 9 patients with chronic periodontitis and herpetiform stomatitis in the postcovid period (group II), 11 conditionally healthy young people (group III, comparisons). The biomaterial was selected after professional oral hygiene from periodontal spaces; metagenomic analysis was carried out using a Nanodrop ND-2000 spectrophotometer, “Quant-iT dsDNA HS” analysis kits and “MiSeq” sequencer (Illumina). >. The structures of microbial communities of 28 samples of the microbiome of periodontal spaces were analyzed, the average length of the resulting sequences was 460 base pairs, an average of 13 974 sequences accounted for the sample, 183 phylotypes belonging to 17 types were identified, of which 46 of the most numerous phylotypes were identified. Relative to the comparison group, the experimental groups showed a tendency to decrease the normal microbiota (Streptococcus, Rothia), an increase in the biodiversity of Shannon and individual representatives of the conditionally pathogenic spectrum (group I — Prevotella, Fusobacterium, Campylobacter, Haemophilus, Selenomonas, Treponema, Tannerella and Filifactor; group II — unclassified TM7-3, Actinomyces, Veillonella, unclassified Gemellaceae, Porphyromonas). >. The features of the microbiome of the periodontal pocket in comorbid patients with periodontitis and herpes in the postcovid period were revealed, individual representatives of the microbiome were identified, their features were described, which gives grounds for the use of various treatment methods, including using minimally invasive therapy against the background of antiviral agents.