DOI:

10.37988/1811-153X_2022_4_26

Antibacterial efficiency of anodic dissolution of various types of electrodes used for direct current trans-channel effects

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Authors

  • N.Zh. Dikopova 1, PhD in Medical Sciences, associate professor of the Therapeutic dentistry Department
    ORCID: 0000-0002-4031-2004
  • A.V. Tsarev 2, postgraduate at the Dentistry diseases propaedeutics Department
    ORCID: 0000-0002-1900-0962
  • E.V. Ippolitov 3, PhD in Medical Sciences, professor of the Microbiology, virology, immunology Department
    ORCID: 0000-0003-1737-0887
  • A.G. Volkov 1, PhD in Medical Sciences, professor of the Therapeutic dentistry Department
    ORCID: 0000-0003-2674-1942
  • S.N. Razumova 2, PhD in Medical Sciences, associate professor and head of the Dentistry diseases propaedeutics Department
    ORCID: 0000-0002-9533-9204
  • M.S. Podporin 3, PhD in Medical Sciences, researcher at the Molecular biology research Laboratory of the Medico-dental research Institute
    ORCID: 0000-0001-6785-0016
  • 1 Sechenov University, 119991, Moscow, Russia
  • 2 RUDN University, 117198, Moscow, Russia
  • 3 Moscow State University of Medicine and Dentistry, 127473, Moscow, Russia

Abstract

Today in endodontics, the problem of disinfection of obliterated canals remains unresolved. In the treatment of such teeth, transcanal effects with direct current are used. The aim of the work was to study the antioxidant efficiency of anodic dissolution of silver-copper and copper electrodes, cases of apical effects on the immune system, in relation to pathogenic risks, received from root canals when examining chronic pulpitis. >. An experimental microbiological study was carried out using automatic cultivation in liquid nutrient media. For the study, were used strains of bacteria and yeast fungi obtained from the root canals of teeth in the treatment of chronic pulpitis: S. constellatus, P. intermedia, C. albicans. To study the antibacterial effectiveness of the anodic dissolution of the silver-copper electrode, 2 silver-copper electrodes used in apexphoresis were placed in a test tube. When studying the antibacterial efficiency of the anodic dissolution of the copper electrode, 2 copper electrodes were placed in test tubes, which were stripped of insulation, placed in test tubes and connected to a current source: plus at the bottom of the tube, minus at the surface. Based on the data obtained, growth curves of bacterial and fungal populations were constructed for each experiment with registration of points of change in optical density in periods of adaptation, accelerated growth, exponential growth, inhibition of development, stationary equilibrium, culture withering away. >. Anodic dissolution of both silver-copper and copper electrodes has a pronounced and, in general, unidirectional antibacterial effect. A significant decrease in optical density relative to the control sample was noted: for S. constellatus in the sample Ag+Cu — by 55.66%, in the sample Cu — by 63.64%; for P. intermedia in the sample Ag+Cu — by 68.6%, in the sample Cu — by 69.44%; for C. albicans in the sample Ag+Cu by 54.09%, in the sample Cu by 68.98%. >. It was found that if anodic dissolution of a copper electrode has a certain advantage for some representatives of the microbiota, then for P. intermedia it is more effective to use a silver-copper electrode, which confirms the possibility of using anodic dissolution of a silver-copper copper and copper electrodes.

Key words:

trans-channel effects, direct current, apex-phoresis, anodic dissolution, electrode, antibacterial efficiency

For Citation

[1]
Dikopova N.Zh., Tsarev A.V., Ippolitov E.V., Volkov A.G., Razumova S.N., Podporin M.S. Antibacterial efficiency of anodic dissolution of various types of electrodes used for direct current trans-channel effects. Clinical Dentistry (Russia).  2022; 25 (4): 26—33. DOI: 10.37988/1811-153X_2022_4_26

References

  1. Razumova S.N., Timohina M.I., Bulgakov V.S., Anurova A.E. The factors that ensure quality endodontic treatment. Health and Education Millennium. 2015; 2: 35—36 (In Russ.). eLIBRARY ID: 23239245
  2. Razumova S.N., Brago A.S., Barakat H., Khaskhanova L.M., Bragunova R.M. Assessment of the results of endodontic treatment of teeth. Endodontics Today. 2020; 1: 27—30 (In Russ.). eLIBRARY ID: 43167054
  3. Razumova S.N., Selifanova E.I., Manveljan A.S., Fomina A.D., Alsekhnaui R. Experience of combined treatment of the chronic apical periodontitis implementing surgical procedures. Endodontics Today. 2017; 2: 55—58 (In Russ.). eLIBRARY ID: 29969386
  4. Razumova S., Brago A., Khaskhanova L., Barakat H., Howijieh A. Evaluation of anatomy and root canal morphology of the maxillary first molar using the cone-beam computed tomography among residents of the Moscow region. Contemp Clin Dent. 2018; 9 (Suppl 1): S133-S136. PMID: 29962778
  5. Razumova S., Brago A., Khaskhanova L., Howijieh A., Barakat H., Manvelyan A. A Cone-beam computed tomography scanning of the root canal system of permanent teeth among the Moscow Population. Int J Dent. 2018; 2018: 2615746. PMID: 30356403
  6. Razumova S.N., Brago A.S., Khaskhanova L.M., Barakat Kh.B., Khuayzhi A., Manvelyan A.S. Anatomy of the root canal system of the mandible teeth according to cone-beam computed tomography. Endodontics Today. 2018; 4: 50—52 (In Russ.). eLIBRARY ID: 37064698
  7. Daurova F.Yu., Volkov A.G., Dikopova N.Zh., Tomaeva D.I., Arzukanyan A.V. The effectiveness of high-frequency monopolar diathermocoagulation in the treatment of chronic pulpitis. Endodontics Today. 2019; 2: 36—40 (In Russ.). eLIBRARY ID: 39322270
  8. Makeeva I.M., Volkov A.G., Dikopova N.Zh., Talalaev E.G. Endodontic treatment efficacy enhancement by means of instrumental physiotherapy. Stomatology. 2017; 2: 17—19 (In Russ.). eLIBRARY ID: 29243446
  9. Razumova S., Brago A., Barakat H., Howijieh A., Senyagin A., Serebrov D., Guryeva Z., Kozlova Y., Adzhieva E. Evaluation of the microbiological effect of colloidal nanosilver solution for root canal treatment. J Funct Biomater. 2022; 13 (4): 10. PMID: 36278632
  10. Razumova S., Brago A., Serebrov D., Barakat H., Kozlova Y., Howijieh A., Guryeva Z., Enina Y., Troitskiy V. The application of nano silver argitos as a final root canal irrigation for the treatment of pulpitis and apical periodontitis. In vitro study. Nanomaterials (Basel). 2022; 12 (2): 10. PMID: 35055265
  11. Rumyantsev V.A., Frolov G.A., Blinova A.V., Karasenkov Y.N., Bityukova E.V. Electron microscopic properties of a new antimicrobial nanodrug based on copper-calcium hydroxide compound. Avicenna Bulletin. 2021; 4: 532—541 (In Russ.). eLIBRARY ID: 48042741
  12. Yefanov O.I., Tsaryov V.N., Nosik A.S., Volkov A.G., Dikopova N.Zh. In vitro study of the antibacterial activity of apex-phoresis using silver-copper electrode. Russian Journal of Dentistry. 2006; 4: 1—6 (In Russ.). eLIBRARY ID: 9247571
  13. Razumova S., Brago A., Kozlova Yu., Barakat H., Howijieh A. Evaluation the efficacy of Er: YAG laser in removing the smear layer during endodontic treatment. Journal of International Dental and Medical Research. 2021; 14 (3): 933—937.
  14. Kopytov A.A., Kuzmina E.A., Borozentseva V.A., Razumova S.N., Brago A.S., Rokhoeva M.S. The effect of the quality of removal of temporary sealers from anatomically limited volumes of the root canal system on the quality of root canal obturation. Medical alphabet. 2022; 2: 11—16 (In Russ.). eLIBRARY ID: 48289087
  15. Yefanov O.I., Tsaryov V.N., Volkov A.G., Nikolayeva Ye.N., Nosik Nosika., Dikopova N.Zh. The antibacterial efficacy of different kinds of transcanal direct current application. Russian Journal of Dentistry. 2008; 2: 38—42 (In Russ.). eLIBRARY ID: 11576112
  16. Daurova F.Yu., Volkov A.G., Dikopova N.Zh., Nosik A.S., Tomaeva D.I., Kodzaeva E.S., Arzukanyan A.V. Antibacterial efficacy of various methods of heat treatment of root canals. Pediatric Dentistry and Profilaxis. 2019; 3 (71): 32—36 (In Russ.). eLIBRARY ID: 41197092
  17. Volkov A.G., Daurova F.Yu., Dikopova N.J., Tomaeva D.I., Kodzaeva E.S., Arzukanyan A.V. Diathermocoagulation: substantiation of application in endodontic treatment. International Dental Review. 2018; 4: 32—35 (In Russ.). eLIBRARY ID: 36552839
  18. Volkov A.G., Dikopova N.Zh., Amanatidi G.E., Volkov N.A., Nikolskaya I.A., Eremin D.A., Guseva O.Yu., Tursumatov Zh.A., Pobozhieva L.V., Shevelyuk Yu.V. The results of the frequency of detection of galvanic couple of metal structures in of the oral mucosal diseases. Medical alphabet. 2022; 7: 27—30 (In Russ.). eLIBRARY ID: 48779661
  19. Saygin D., Tabib T., Bittar H.E.T., Valenzi E., Sembrat J., Chan S.Y., Rojas M., Lafyatis R. Transcriptional profiling of lung cell populations in idiopathic pulmonary arterial hypertension. Pulm Circ. 2020; 10 (1): 10. PMID: 32166015
  20. Makeeva I.M., Volkov A.G., Dikopova N.Zh., Ruchkin D.N., Talalaev E.G., Kocharyan A.M. The determination of optimal parameters of electric current for electric pulp testing. International Dental Review. 2018; 2: 20—23 (In Russ.). eLIBRARY ID: 35235185
  21. Makeeva I.M., Volkov A.G., Dikopova N.Z., Makarenko N.V. The determination of electrochemical potentials in the oral cavity, as a way to diagnose galvanic syndrome, which contributes to the development of diseases of the mucous membrane. Head and Neck. 2018; 1: 42—45 (In Russ.). eLIBRARY ID: 41304588
  22. Volkov A.G., Prikuls V.F., Dikopova N.Zh., Nosik A.S., Arzukanyan A.V. The study on the impact of various types of currents on root canal microbiota. Stomatology. 2019; 2: 37—41 (In Russ.). eLIBRARY ID: 37785545
  23. Razumova S., Brago A., Howijieh A., Barakat H., Kozlova Yu., Razumov N. Evaluation the relationship between mandibular molar root apices and mandibular canal among residents of the Moscow population using cone-beam computed tomography technique. Contemporary Clinical Dentistry. 2022; 13 (1): 3—8. DOI: 10.4103/ccd.ccd_388_19
  24. Daurova F.Yu., Volkov A.G., Dikopova N.Zh., Tomaeva DianaI., Kodzaeva E.S. Applying high frequency monopolar diathermocoagulation of the endodontic treatment of teeth. Russian Journal of Dentistry. 2018; 2: 117—120 (In Russ.). eLIBRARY ID: 35419626
  25. Yefanov O.I., Volkov A.G., Nosov V.V. The distribution of copper and silver in dental root tissues during apex-phoresis, and the degree of root canal permeability. Russian Journal of Dentistry. 2008; 5: 7—10 (In Russ.). eLIBRARY ID: 11679017
  26. Efanov O.I., Tsarev V.N., Volkov A.G., Nosik A.S., Dikopova N.Zh., Shpilko A.L., Tret’yakov A.A. Antibaterial effects of zinc during apex-phoresis. Russian Journal of Dentistry. 2012; 1: 5—9 (In Russ.). eLIBRARY ID: 18112380
  27. Efanov O.I., Volkov A.G. Efficiency and prospects of development of transchannel influences by a direct current at treatment of teeth with almost impassable root channels. Orthodontics. 2009; 3 (47): 32—37 (In Russ.). eLIBRARY ID: 18764684
  28. Volkov A.G., Dikopova N.Zh., Shpilko A.L. Transcanal direct current and laser-magnet therapy for treating teeth with diffi cult root canals. Laser Medicine. 2011; 2: 101-a (In Russ.). eLIBRARY ID: 22628139
  29. Volkov A.G., Dikopova N.Zh., Arzukanyan A.V., Kondratiev S.A., Paramonov Yu.O., Budina T.V., Tan H. Distribution of metal compounds in the tissues of the root of the tooth with apex-foreses (Iontophoresis of copper and silver). New Armenian Medical Journal. 2021; 1 (15): 59—66.

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Received

July 14, 2022

Accepted

December 6, 2022

Published on

December 21, 2022