DOI:

10.37988/1811-153X_2020_3_4

Estimation of human saliva structurization at dehydration on the solid substrate

Downloads

Authors

  • A.K. Martusevich 1, PhD in Biology, head of the Medical biophysics laboratory at the University hospital; professor of the Animal physiology and biochemistry Department
  • L.K. Kovaleva 2, PhD in Biology, assistant professor of the Histology and embryology department
  • L.M. Kozlova 1, PhD candidate at the Medical biophysics Laboratory of the University hospital
  • A.N. Tuzhilkin 1, research lab technician of the Medical biophysics laboratory at the University hospital; student
  • A.S. Fedotova 3, student
  • S.Yu. Krasnova 1, junior researcher of the Medical biophysics laboratory at the University hospital
  • 1 Privolzhsky Research Medical University, 603005, Nizhniy Novgorod, Russia
  • 2 Kuban State Medical University, 350063, Krasnodar, Russia
  • 3 Nizhny Novgorod State Agricultural Academy, 603107, Nizhny Novgorod, Russia

Abstract

The aim of this work is specification of biophysical technologies in estimation of human saliva dehydration structurization. Materials and methods. We study saliva specimens by 95 healthy 24—27 years old adults. Specialties of own and initiated (by 0.1%, 0.9% and 10% solutions of sodium chloride; 0.1 N hydrochloric acid solution; 0.01 N potassium hydroxide solution) saliva structurization at dehydration on hard padding were estimated with system of morphometric parameters and spectrometry investigation. The range of estimated parameters of crystallography included: crystallizability, structure index, type of interaction of crystalline and amorphous structures, facies destruction degree, uniformity of crystal distribution, expression of cellular structure, edge zone and other facies zones. To describe teziographic facies we used: main teziographic coefficient, belts coefficient crystallinity, and the rest parameters are the same ones for crystalloscopy. We used 3 wave lengths (300, 350 and 400 nm) for spectrometry of dehydrated specimens. Role of saliva temperature, pH and osmolarity in biological fluid structurization was fixed. Results. Teziocrystalloscopic and spectrometric patterns of dehydration of samples of oral fluid of healthy humans was established according to a new system of evaluation criteria. It was stated, that saliva dehydration is dynamic process of its liquid component elimination, determined by its composition. Micro- and macro-environment factors (temperature, pH, and osmolarity) affect on saliva dehydration results considerably.

Key words:

saliva, structurization, dehydration, biocrystallomics

For Citation

[1]
Martusevich A.K., Kovaleva L.K., Kozlova L.M., Tuzhilkin A.N., Fedotova A.S., Krasnova S.Yu. Estimation of human saliva structurization at dehydration on the solid substrate. Clinical Dentistry (Russia).  2020; 3 (95): 4—9. DOI: 10.37988/1811-153X_2020_3_4

References

  1. Korotko G.F. Secretion of salivary glands and salivadiagnostics elements. — Moscow: Academy of Natural History, 2006. — P. 4—10 (In Russ.).
  2. Barer G.M., Denisov A.B. Crystallographic method of saliva study. — Moscow, 2008. — P. 15—26 (In Russ.).
  3. Artishevsky A.A., Gaiffullina B., Malkovets O.G. Crystallization of saliva aggregates in different phases of ovarian menstrual cycle. — Sovremennaya stomatologiya (Belarus). — 2006; 4: 74—7 (In Russ.).
  4. Gavrilova O.A. Quantitative characteristics of physical and chemical properties of oral fluid in preschool children. — Stomatology. — 2004; 83 (2): 54—6 (In Russ.).
  5. Denisov A.B., Pushkar’ D.Y., Denisov S.A. Use of saliva crystallogenic properties for early diagnostics of prostate cancer. — Bull Exp Biol Med. — 2006; 142 (2): 242—5. PMID: 17369950
  6. Antropova I.P., Gabinsky Ya.L. Crystallization of biological fluid in close cell on saliva example. — Russian Clinical Laboratory Diagnostics Journal. — 1997; 8: 36—8 (In Russ.).
  7. Shabalin V.N., Rasumova S.N., Uvarova D.S. Age dynamics of oral liquid chemical elements composition. — Russian stomatology journal. — 2014; 2: 41—3 (In Russ.).
  8. Shabalin V.N., Shatokhina S.N. Morphology of human biological fluids. — Moscow: Chryzostom, 2001. — P. 58—120 (In Russ.).
  9. Vorobyov A.V., Martusevich A.K., Peretyagin S.P. Crystallogenesis of biological fluids and substrates in the assessment of the state of the body. — Nizhny Novogorod, 2008. — P. 4—17 (In Russ.).
  10. Denisov A.B. Saliva microcrystallization: new methodical approaches. — Stomatology. — 2007; 86 (5): 20—3 (in Russ.).
  11. Jordanishvili A.K. Oral liquid adult: age peculiarities of the physicochemical properties and micro crystallization. — Adv Gerontol. — 2019; 32 (3): 477—82 (In Russ.).
  12. Redinova T.L. Microcrystallization of the saliva in children following carbohydrate intake and the performance of caries prophylactic measures. — Stomatologiia (Mosk). — 1989; 68 (4): 62—3 (in Russ.).
  13. Pancu G., Lăcătuşu S., Căruntu I.D., Iovan G., Ghiorghe A. Evaluation of caries activity using the micro-crystallization saliva index (IMK). — Rev Med Chir Soc Med Nat Iasi. — 2006; 110 (1): 206—11 (In Romanian). PMID: 19292107
  14. Kovalenko V.V., Tkachenko I.M., Nazarenko Z.Y., Brailko N.M., Romanova J.G., Sheshukova O.V., Vodoriz Y.V. The study of oral fluid dynamic parameters on the background of pathological and physiological dental abrasion. — Wiad Lek. — 2019; 72 (7): 1315—9. PMID: 31398162
  15. Iijima M., Hashimoto M., Kohda N., Nakagaki S., Muguruma T., Endo K., Mizoguchi I. Crystal growth on bioactive glass sputter-coated alumina in artificial saliva. — Dent Mater J. — 2013; 32 (5): 775—80. PMID: 24088833
  16. Deryabina N.I., Zalessky M.G. the Content of protein components in a drop of blood serum when it dries. — Journal of new medical technologies. — 2005; 12 (1): 85—7 (In Russ.).
  17. Yakhno T.A., Yakhno V.G., Sanin A.G., Sanina O.A., Pelyushenko A.S. Protein and salt: spatiotemporal dynamics of events in a drying drop. — Technical Physics. The Russian Journal of Applied Physics. — 2004; 74 (8): 100—8 (In Russ.).
  18. Kolodkina E.V., Kamakin N.F. A procedure for determining the proteolytic activity of biological substrates at various pH values. — Klin Lab Diagn. — 2008; (7): 16—7 (In Russ.).
  19. Martusevich A.K., Kamakin N.F. Crystallography of biological fluid as a method for evaluating its physicochemical characteristics. — Bull Exp Biol Med. — 2007; 143 (3): 385—8. PMID: 18225770

Downloads

Published on

September 15, 2020