DOI:

10.37988/1811-153X_2023_3_125

Experimental microbiological justification of disinfection measures as a component of infectious safety in the practice of an orthopedic dentist

Downloads

Authors

  • V.N. Tsarev 1, PhD in Medical Sciences, full professor of the Microbiology, virology, immunology department, director of the Medico-dental research Institute
    ORCID: 0000-0002-3311-0367
  • A.N. Akavov 2, postgraduate at the Prosthodontics Department
    ORCID: 0009-0000-6987-6308
  • V.M. Karpova 1, PhD in Medical sciences, associate professor of the Dentistry diseases propaedeutics Department
    ORCID: 0000-0003-1003-6667
  • E.V. Tsareva 1, PhD in Medical Sciences, assistant professor of the Dentistry diseases propaedeutics Department
    ORCID: 0000-0001-8600-0447
  • A.A. Lastochkin 1, PhD in Medical Sciences, associate professor of the Microbiology, virology, immunology Department
    ORCID: 0009-0006-0473-4791
  • 1 Moscow State University of Medicine and Dentistry, 127473, Moscow, Russia
  • 2 Dagestan State Medical University, 367012, Makhachkala, Russia

Abstract

It is estimated that, on average, 6 to 9% of hospitalized patients suffer from the development and severe course of infectious complications caused by healthcare-associated pathogens each year. Solving the problems of infection safety, including in the practical work of a dentist, is an extremely urgent problem. The aim of the study was to experimentally substantiate the choice of preparations with antibiofilm action for optimizing the disinfection of dental impressions.
Materials and methods.
The microbiota of 34 silicone dental impressions was analyzed. Subsequently, the effect of preparations from the group of quaternary ammonium compounds (QAC) recommended for chemical disinfection (benzalkonium chloride, cetylpyridinium chloride, and benzyldimethyl[3-(myristoylamino)propyl]ammonium chloride) on the experimental biofilm was evaluated in an in vitro experiment using a scanning electron microscope.
Results.
The most significant level of microbial contamination exceeding the upper limit of known oral norms (104±102 CFU/mL) was registered for Streptococcus spp., S. sanguis, Corynebacterium spp., Fusobacterium spp., Enterococcus spp., Porphyromonas gingivalis (in the range of 105—107 CFU/mL). This made it possible to identify priority pathogens for the experiment of mixed biofilm formation and visual control of its destruction using QAC. The proposed experimental models can be used to select the optimal antiseptic in the range of 0.05—0.1%. Complete eradication of experimental biofilm pathogens and planktonic forms occurred when 0.1% solutions of biocidal agents were applied.
Conclusion.
QAC causes destruction of experimental biofilm. Miramistin at a concentration of 0.05% showed more pronounced activity against the strains of anaerobic microbiota studied.

Key words:

infection complication, disinfection by immersion, dental impressions, quaternary ammonium derivatives, miramistin

For Citation

[1]
Tsarev V.N., Akavov A.N., Karpova V.M., Tsareva E.V., Lastochkin A.A. Experimental microbiological justification of disinfection measures as a component of infectious safety in the practice of an orthopedic dentist. Clinical Dentistry (Russia).  2023; 26 (3): 125—133. DOI: 10.37988/1811-153X_2023_3_125

References

  1. Kovaleva E.P. Socio-economic significance of nosocomial infections. In: Labinskaya A.S., Volina E.G., Kovaleva E.P. (eds.) Handbook of Medical microbiology. Book III, vol. 2. Opportunistic infections: clinical and epidemiological aspects. Moscow: Binom, 2014. Pp. 21—34 (In Russian).
  2. Order of the Ministry of Health of the Russian Federation No. 1108n “On approval of the procedure for preventive measures, detection and registration in a medical organization of cases of infectious diseases associated with the provision of medical care that are subject to detection and registration in a medical organization”, November 29, 2021 (In Russian).
  3. Sanitary and epidemiological requirements for the prevention of infectious diseases. Sanitary rules and regulations 3.36-86-21 (In Russian).
  4. Sterilization of medical devices. Microbiological methods. Part 2. Sterility tests carried out during the validation of sterilization processes. Interstate standard GOST ISO 11737-2-2011, effective from 2013.01.01 (In Russian).
  5. Orlova O.A., Tutelyan A.V., Zamyatin M.N., Akimkin V.G. Epidemiological diagnosis of infections associated with provision of medical care at current state. Medical alphabet. 2019; 32 (407): 5—10 (In Russian). eLIBRARY ID: 42386061
  6. Arutyunov S.D., Khsigova Z.V., Kamilov R.I., Tsarev V.N., Ippolitov E.V. Comparative evaluation of the impact of new chemical disinfectants to physical and microbiological characteristics dental impressions. Russian Journal of Dentistry. 2013; 5: 34—38 (In Russian). eLIBRARY ID: 21184249
  7. Khrapunova I.A., Shestopalov N.V. The role of disinfection measures in the prevention of infections associated with the provision of medical care teased on the materials of new regulatory documents). Disinfection Affairs. 2022; 2 (120): 30—37 (In Russian). eLIBRARY ID: 48659621
  8. Rutala W.A., Weber D.J. Guideline for disinfection and sterilization in healthcare facilities. Atlanta (USA): CDC, 2019. Pp. 240—264.
  9. Haque M., Sartelli M., McKimm J., Abu Bakar M. Health care-associated infections an overview. Infect Drug Resist. 2018; 11: 2321—2333. PMID: 30532565
  10. Rutala W.A., Weber D.J. Disinfection and sterilization in health care facilities: An overview and current issues. Infect Dis Clin North Am. 2016; 30 (3): 609—37. PMID: 27515140
  11. Ofstead C.L., Wetzler H.P., Snyder A.K., Horton R.A. Endoscope reprocessing methods: a prospective study on the impact of human factors and automation. Gastroenterol Nurs. 2010; 33 (4): 304—11. PMID: 20679783
  12. Kryukov E.V., Cherkashin D.V., Reutskiy I.A., Solntsev V.N., Bucenko S.A., Sobolev A.D., Levankov B.V., Dmitriev M.V., Efimov S.V., Kutelev G.G. Differentiated approach to the implementation of preventive and anti-epidemic measures among military personnel based on the COVID-19 disease risk assessment scale. Infectious Diseases: News, Views, Education. 2021; 2 (37): 31—38 (In Russian). eLIBRARY ID: 46275696
  13. Pankratova G.P., Bidevkina M.V. Application of disinfectants in the food and processing industry. Disinfection Affairs. 2022; 2 (120): 5—11 (In Russian). eLIBRARY ID: 48659618
  14. Bernhardt A., Wehrl M., Paul B., Hochmuth T., Schumacher M., Schütz K., Gelinsky M. Improved sterilization of sensitive biomaterials with supercritical carbon dioxide at low temperature. PLoS One. 2015; 10 (6): e0129205. PMID: 26067982
  15. Tsarev V.N., Pokrovsky V.N., Lastochkin A.A., Zavadsky R.V., Nikolaeva E.N. Fundamentals of microbiology and disinfection. Moscow: MSMDU, 2017. 85 p. (In Russian).
  16. Pereverzeva E.V., Melnichuk V.I. Disinfection. Sterilization: Methodological recommendations. Minsk: BSMU, 2019. 16 p. (In Russian).
  17. Arutyunov S.D., Yanushevich O.O., Korsunsky A.M., Podporin M.S., Salimon I.A., Romanenko I.I., Tsarev V.N. Comparative analysis of the effectiveness of modern methods of sterilization of instruments and the place of gas-dynamic treatment with carbon dioxide. Russian Stomatology. 2022; 1: 12—19 (In Russian). eLIBRARY ID: 48470527
  18. Salimon A.I., Statnik E.S., Kan Yu., Yanushevich O.O., Tsarev V.N., Podporin M.S., Arutyunov S.D., Skripnichenko P.Yu., Galstyan M.S., Korsunsky A.M. Comparative study of biomaterial surface modification due to subcritical CO2 and autoclave disinfection treatments. The Journal of Supercritical Fluids. 2022; 191: 105789. DOI: 10.1016/j.supflu.2022.105789
  19. Zverev V.V., Boychenko M.N. Medical microbiology: textbook for medical schools. Moscow: GEOTAR-Media, 2023. Pp. 137—170. (In Russian).
  20. Tsarev V.N. Laboratory diagnostics of anaerobic (non-clostridial) infection. In: Labinskaya A.S., Volina E.G., Kovaleva E.P. (eds.) Handbook of Medical microbiology. Book III, vol. 1. Opportunistic infections: clinical and epidemiological aspects. Moscow: Binom, 2013. Pp. 439—453 (In Russian).
  21. Ippolytov E.V. Monitoring of microbial biofilm formation and optimization of the diagnosis of inflammatory periodontal diseases: dissertation abstract. Moscow: Sechenov University, 2016. 48 p. (In Russian).
  22. Tsarev V.N., Ippolitov E.V., Nikolaeva E.N. Prevalence of genetic markers of resistance to antibiotics in biofilm-forming strains of obligate and elective anaerobes. Journal of Microbiology, Epidemiology and Immunobiology. 2017; 2: 74—80 (In Russian). eLIBRARY ID: 37330862
  23. Frolov G.A., Abdukadirov A.A., Lundovskikh I.A., Mironina A.Y., Gurin K.I., Pogorelsky I.P., Mironin A.V., Gavrilov K.E. Experimental evaluation of Yersinia pseudotuberculosis bacteria sensitivity to antimicrobial compositions based on copper nanoparticles in in vitro and in vivo experiments. Disinfection Affairs. 2023; 1 (123): 5—23 (In Russian). eLIBRARY ID: 50451660
  24. Ippolitov E.V., Tsarev V.N., Avtandilov G.A., Tsareva E.V., Didenko L.V. Microbial biofilms on the surface of dental polymeric materials as the main factor of microbial persistence in dental and periodontal pathology. Russian Stomatology. 2016; 1: 92—93 (In Russian). eLIBRARY ID: 26005922
  25. Ivanov S.Yu., Karasenkov Ya.N., Latuta N.V., Dzhatdaev V.V., Egorov E.A., Tarasova E.K., Kozlova E.V., Kozlov P.A. Nanotechnology in dentistry: hydrosols of metal nanoparticles are promising antibiotics. Clinical Dentistry (Russia). 2023; 1: 158—163 (In Russian). eLIBRARY ID: 50465583
  26. Losev F.F., Smirnova L.E. Assessment of functional areas of activity in a medical organization in the context of the introduction of a quality control system and safety of medical activities. Clinical Dentistry (Russia). 2022; 3: 126—131 (In Russian). eLIBRARY ID: 49514214

Downloads

Received

July 27, 2023

Accepted

August 7, 2023

Published on

September 24, 2023