DOI:

10.37988/1811-153X_2023_1_60

Bactericidal activity of saliva during cryotherapy and ozone therapy of verrucous leukoplakia of the oral mucosa

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Authors

  • S.I. Tokmakova 1, PhD in Medical Sciences, full professor of the Therapeutic dentistry Department
    ORCID: 0000-0003-0437-0079
  • K.V. Ziablitskaia 1, assistant at the Therapeutic dentistry Department
    ORCID: 0000-0002-6451-9421
  • O.V. Bondarenko 1, PhD in Medical Sciences, associate professor of the Therapeutic dentistry Department
    ORCID: 0000-0001-7280-7709
  • V.V. Prokopev 1, PhD in Biological Sciences, assistant professor of the Epidemiology, Microbiology and Virology Department
    ORCID: 0000-0001-7151-3899
  • Yu.V. Lunitsyna 1, PhD in Medical Sciences, associate professor of the Therapeutic dentistry Department
    ORCID: 0000-0002-2442-3361
  • E.V. Mokrenko 2, PhD in Medical Sciences, full professor of the Prosthodontics Department
    ORCID: 0000-0002-4286-3993
  • Yu.Yu. Gurevich 1, PhD in Medical Sciences, associate professor of the Prosthodontics Department
    ORCID: 0000-0003-1280-0702
  • E.S. Zhukova 1, PhD in Medical Sciences, associate professor of the Therapeutic dentistry Department
    ORCID: 0000-0002-8578-4232
  • D.S. Bondarenko 1, 3rd year student at the Institute of Clinical Medicine
    ORCID: 0000-0001-8601-9930
  • 1 Altai State Medical University, 656038, Barnaul, Russia
  • 2 Irkutsk State Medical University, 664003, Irkutsk, Russia

Abstract

Leukoplakia of the oral mucosa has a high risk of malignancy. One of the options for radical treatment is cryosurgery. The healing of the wound depends on the method of postoperative management and the properties of the oral fluid. The use of ozonated olive oil with a high redox potential in this period provides anti-inflammatory, analgesic and antimicrobial effects. The aim is to evaluate the effectiveness of the use of ozonated oil in patients with verrucous leukoplakia after cryosurgical removal of foci. >. 62 patients with leukoplakia were treated by cryodestruction with liquid nitrogen. In the postoperative period, ozonated oil was applied to the wound in patients of the main group, rinsing with chlorhexidine (0.06%) was applied to the comparison group. We used a comparative analysis of the antibacterial activity of saliva from 1 to 7 days after cryodestruction by changing the optical density of filtrates (ODF) of oral fluid. >. The studied indicators of OPF of opportunistic microorganisms in the group with ozone therapy were significantly lower compared to traditional treatment during 3 hours of incubation all study periods. At all points of measurement, the ODF of Streptococcus pneumoniae varied from 0.081 to 0.096; Escherichia coli — from 0.079 to 0.198; Staphylococcus aureus — from 0.082 to 0.143, Pseudomonas aeruginosa — from 0.091 to 0.104. It was significantly lower than the control by an average of 20% and less than in the comparison group. In Candida albicans filtrates, a similar trend of ODF differences was traced. >. The revealed lower optical density of oral fluid filtrates in the group with ozone therapy throughout the incubation period indicates the preservation of a more pronounced antibacterial effect of saliva under the action of ozonated oil for 3 hours after application compared with chlorhexidine solution. It allows to justify the prolonged antiseptic effect of ozone therapy in the complex treatment after cryodestruction of oral verrucous leukoplakia.

Key words:

verrucous leukoplakia, ozone therapy, oral microbiocenosis, oral fluid, bactericidal activity

For Citation

[1]
Tokmakova S.I., Ziablitskaia K.V., Bondarenko O.V., Prokopev V.V., Lunitsyna Yu.V., Mokrenko E.V., Gurevich Yu.Yu., Zhukova E.S., Bondarenko D.S. Bactericidal activity of saliva during cryotherapy and ozone therapy of verrucous leukoplakia of the oral mucosa. Clinical Dentistry (Russia).  2023; 26 (1): 60—67. DOI: 10.37988/1811-153X_2023_1_60

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Received

December 28, 2022

Accepted

January 27, 2023

Published on

March 22, 2023