DOI:

10.37988/1811-153X_2022_4_74

Experimental substantiation of the development of the dosage form of lactoferrin with enamel matrix derivatives for use in periodontology

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Authors

  • M.S. Podporin 1, PhD in Medical Sciences, researcher at the Molecular biology research Laboratory of the Medico-dental research Institute
    ORCID: 0000-0001-6785-0016
  • V.N. Tsarev 1, PhD in Medical Sciences, director of the Medico-dental research Institute
    ORCID: 0000-0002-3311-0367
  • E.V. Ippolitov 1, PhD in Medical Sciences, leading researcher at the Molecular biology research Laboratory of the Medico-dental research Institute
    ORCID: 0000-0003-1737-0887
  • T.V. Tsareva 1, PhD in Medical Sciences, associate professor of the Microbiology, virology, immunology Department
    ORCID: 0000-0001-9571-0520
  • V.V. Vishlenkova 1, 4th year student at the Medical Faculty
    ORCID: 0000-0001-6065-1997
  • I.L. Goldman 2, PhD in Medical Sciences, leading researcher
    ORCID: 0000-0003-1534-3367
  • E.R. Sadchikova 2, PhD in Chemical Sciences, deputy director
    ORCID: 0000-0003-2039-7108
  • 1 Moscow State University of Medicine and Dentistry, 127473, Moscow, Russia
  • 2 Institute of Gene Biology of the Russian Academy of Sciences, 119334, Moscow, Russia

Abstract

When performing reconstructive operations in periodontology, the use of a derivative of the enamel matrix Emdogaine, which stimulates the regeneration of soft tissues well, is promising, but it is not always effective enough against infectious inflammation. Lactoferrin is considered among the possible candidate drugs that have an anti-inflammatory antimicrobial effect. The aim of the study was to experimentally substantiate the antimicrobial action of lactoferrin in combination with enamel matrix derivatives in the formation of a depot of a complex drug. >. To assess the antimicrobial activity of the Emdogaine and lactoferrin complex, programmed automatic cultivation of S. aureus was performed. The results were recorded non-invasively, using the built-in optical density measurement system, in accordance with the key points of culture development in the periodic cultivation system. Statistical processing was carried out by constructing a regression dependence (a second-order parabola), with an estimate of the Fisher criterion and the Pearson correlation coefficient. Results and discussion. When cultivating S. aureus with Emdogaine, there was no statistically significant difference in the change in optical density relative to the control sample, compared with both lactoferrin and its combination with osteoplastic material, which demonstrated a pronounced antibacterial effect. The result obtained was statistically reliable. >. The use of lactoferrin in combination with the osteoplastic material Emdogaine leads to a new quality of the complex — prolonged bacteriostatic action against the studied microbial populations and can be recommended for the development of a dosage form with regeneration stimulating and antimicrobial properties.

Key words:

lactoferrin, bioreactor, bone regeneration, periodontitis, enamel matrix, protein

For Citation

[1]
Podporin M.S., Tsarev V.N., Ippolitov E.V., Tsareva T.V., Vishlenkova V.V., Goldman I.L., Sadchikova E.R. Experimental substantiation of the development of the dosage form of lactoferrin with enamel matrix derivatives for use in periodontology. Clinical Dentistry (Russia).  2022; 25 (4): 74—80. DOI: 10.37988/1811-153X_2022_4_74

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Received

November 7, 2022

Accepted

November 15, 2022

Published on

December 21, 2022