DOI:

10.37988/1811-153X_2025_3_112

Comparison of Ti6Al4V titanium alloy membrane prototypes for bone defect repair made by laser sintering and electron beam melting

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Authors

  • A.A. Dolgalev 1, Doctor of Science in Medicine, professor of the General practice and pediatric dentistry Department
    ORCID: 0000-0002-6352-6750
  • H.M. Nalchajyan 2, PhD student of the Maxillofacial surgery and surgical dentistry Department
    ORCID: 0000-0002-6741-4916
  • A.A. Muraev 2, Doctor of Science in Medicine, professor of the Maxillofacial surgery and surgical dentistry Department
    ORCID: 0000-0003-3982-5512
  • Y.S. Petronyuk 3, PhD in Physics and Mathematics, leading researher of the Acoustic microscopy Lab
    ORCID: 0000-0002-9187-5288
  • E.A. Khramtsova 3, junior researcher of the Acoustic microscopy Lab
    ORCID: 0000-0003-1104-8109
  • A.E. Krupnin 4, researcher of the Polymer materials Lab
    ORCID: 0000-0002-5674-4143
  • D.Z. Choniashvili 5, PhD in Medical Sciences, associate professor of the Therapeutic, surgical and pediatric dentistry Department, dean of the Medical faculty
    ORCID: 0000-0003-4218-1359
  • S.Yu. Ivanov 2, 6, Russian Academy of Science corresponding member, Doctor of Science in Medicine, full professor of the Maxillofacial surgery and surgical dentistry Department; full professor of the Maxillofacial surgery Department
    ORCID: 0000-0001-5458-0192
  • 1 Stavropol State Medical University, 355017, Stavropol, Russia
  • 2 RUDN University, 117198, Moscow, Russia
  • 3 Emanuel Institute of Biochemical Physics of the Russian Academy of Sciences, 119334, Moscow, Russia
  • 4 Kurchatov Institute, 123182, Moscow, Russia
  • 5 North Ossetian State University, 362025, Vladikavkaz, Russia
  • 6 Sechenov University, 119991, Moscow, Russia

Abstract

A comparative analysis of the microstructure and mechanical properties of guided bone regeneration frame membranes made from Ti6Al4V titanium alloy powder using 3D printing technology is presented in this paper. Two different methods were used to produce the samples: direct laser sintering of metals (DMLS) and electron beam melting (EBM). The plates, measuring 30×10×1 mm, were formed from layers 30 µm thick. The surface morphology of the samples was studied at both the micro and macro levels using scanning electron microscopy (SEM) and scanning impulse acoustic microscopy (SIAM). Biocompatibility was assessed both in vitro with mesenchymal stem cell (MSC) cultures and in vivo with laboratory animals. Mechanical properties were evaluated using a three-point bending test, which revealed differences in surface profile depth that was 100 and 150 µm for the DMLS and EBM correspondingly. Samples produced using DMLS technology demonstrated higher strength 2,180±20.7 MPa and elasticity 53,449±200 MPa than those produced by EBM strength 1500±26.1 and elasticity 25,633±125 MPa, according to the results of the mechanical tests. A more active proliferation of MSCs was observed in vitro in the DMLS samples, which was 70% higher compared to EMB and the control group. The bone tissue response to both types of titanium implants was good, with high levels of osseointegration, as confirmed by X-ray microtomography (µCT).

Key words:

guided bone regeneration, titanium alloy, laser sintering of metals, Ti6Al4V titanium, dental implant, electron beam melting, porous microstructure

For Citation

[1]
Dolgalev A.A., Nalchajyan H.M., Muraev A.A., Petronyuk Y.S., Khramtsova E.A., Krupnin A.E., Choniashvili D.Z., Ivanov S.Yu. Comparison of Ti6Al4V titanium alloy membrane prototypes for bone defect repair made by laser sintering and electron beam melting. Clinical Dentistry (Russia).  2025; 28 (3): 112—118. DOI: 10.37988/1811-153X_2025_3_112

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Received

April 12, 2025

Accepted

August 14, 2025

Published on

September 21, 2025