DOI:

10.37988/1811-153X_2025_4_50

Dental status and periodontal microbiome in patients with chronic periodontitis and coronavirus infection

Downloads

Authors

  • Z.E. Lalieva 1, postgraduate at the Therapeutic dentistry propaedeutics Department
  • Z.E. Revazova 1, Doctor of Science in Medicine, associate professor of the Therapeutic dentistry propaedeutics Department
  • T.V. Tsareva 1, PhD in Medical Sciences, associate professor of the Microbiology, virology, immunology Department
  • E.V. Tsareva 1, PhD in Medical Sciences, assistant professor of the Therapeutic dentistry propaedeutics Department
  • E.V. Ippolitov 1, Doctor of Science in Medicine, professor of the Microbiology, virology, immunology department
  • 1 Russian University of Medicine, 127006, Moscow, Russia

Abstract

A coronavirus infection can have a negative impact on periodontal tissues, contributing to the development and progression of inflammatory processes due to changes in the composition and structure of the periodontal microbiome. Therefore, the aim of the study was to characterize the dental status and periodontal microbiome in patients with chronic (generalized) periodontitis (CGP) who had a coronavirus infection, as well as to identify the features of the clinical course of the disease and microbiological changes in this group compared to patients who did not have COVID-19.
Materials and methods.
A total of 120 patients diagnosed with CGP were randomized into 2 groups: those with and those without COVID-19, as well as subgroups based on the severity of COVID-19 infection and the severity of CGP. All patients underwent an index assessment of the main indicators of periodontal status, microbiological examination using real-time PCR (PCR-RT) and cultivation for the detection and identification of Candida fungi.
Results.
In the sample of COVID-19 patients, the most common Type I periodontopathogens (PP-1) were P. gingivalis, T. forsythia, A. actinomycetemcomitans and F. alocis (frequency from 64 to 77%), less often T. denticola and P. micra (45 and 27% accordingly), indicating their significant role in the formation of pathogenic microbiota in patients with CGP. C. albicans (48%) and rarer species in C. krusei and C. glabrata (20%) were detected significantly more often than in the group of non-COVID-19 patients. No association was found between the frequency of PP-1 isolation and the severity of CGP, but there was an increase in the isolation of F. alocis and P. micra in patients with progressive pathology (severe periodontitis, moderate and severe COVID-19).
Conclusions.
Contribution of individual microbial species to the development and progression of CGP may vary significantly depending on the presence and severity of the previous coronavirus infection. There was a significant increase in the colonization of the gingival biofilm by P. gingivalis, T. forsythia, A. actinomycetemcomitans, and new species such as F. alocis and P. micra, as well as fungi such as C. albicans, C. krusei, and C. glabrata.

Key words:

chronic periodontitis, periodontal microbiome, COVID-19, periodontopathogens, new species, F. alocis, P. micra, fungi Candida

For Citation

[1]
Lalieva Z.E., Revazova Z.E., Tsareva T.V., Tsareva E.V., Ippolitov E.V. Dental status and periodontal microbiome in patients with chronic periodontitis and coronavirus infection. Clinical Dentistry (Russia).  2025; 28 (4): 50—56. DOI: 10.37988/1811-153X_2025_4_50

References

  1. Yanushevich O.O., Kuzmina E.M., Kuzmina I.N., Lapatina A.V. Trends of oral diseases prevalence among adults in Russia from 1998 to 2018. Medicine and Education. 2024; 1 (17): 17—23 (In Russian). eLIBRARY ID: 82469266
  2. Balmasova I.P., Tsarev V.N., Yanushevich O.O., Maev I.V., Mkrtumyan A.M., Arutyunov S.D. Periodontal microecology. Interrelation of local and systemic effects. Moscow: Practical Medicine, 2021. Pp. 15—21. (In Russian).
  3. Akimkin V.G., Kuzin S.N., et al. Assessment of the COVID-19 epidemiological situation in St. Petersburg. Journal of Microbiology, Epidemiology and Immunobiology. 2021; 5: 497—511 (In Russian). eLIBRARY ID: 47125046
  4. Amkhadova M.A., Petrukhina N.B., Sandler I.V., Polyakov V.M., Demidova A.A., Saltovets M.V. Features of the course of chronic generalized periodontitis after a coronavirus infection. Clinical Dentistry (Russia). 2023; 2: 52—56 (In Russian). eLIBRARY ID: 54167526
  5. Campisi G., Bizzoca M.E., Lo Muzio L. COVID-19 and periodontitis: reflecting on a possible association. Head Face Med. 2021; 17 (1): 16. PMID: 33975613
  6. Gomes S.C., Fachin S., da Fonseca J.G., Angst P.D.M., Lamers M.L., da Silva I.S.B., Nunes L.N. Dental biofilm of symptomatic COVID-19 patients harbours SARS-CoV-2. J Clin Periodontol. 2021; 48 (7): 880—885. PMID: 33899251
  7. Ivanisenko N.V., Seyrek K., Kolchanov N.A., Ivanisenko V.A., Lavrik I.N. The role of death domain proteins in host response upon SARS-CoV-2 infection: modulation of programmed cell death and translational applications. Cell Death Discov. 2020; 6 (1): 101. PMID: 33072409
  8. Gupta S., et al. SARS-CoV-2 detection in gingival crevicular fluid. J Dent Res. 2021; 100 (2): 187—193. PMID: 33138663
  9. Berton F., Rupel K., Florian F., Biasotto M., Pallavicini A., Di Lenarda R. Dental calculus—a reservoir for detection of past SARS-CoV-2 infection. Clin Oral Investig. 2021; 25 (8): 5113—5114. PMID: 34037853
  10. Tsareva T.V., Balmasova I.P., Tsarev V.N. Subgingival microbiome in periodontal disease and comorbid pathology (meta-analysis). Journal of Microbiology, Epidemiology and Immunobiology. 2024; 2: 281—292 (In Russian). eLIBRARY ID: 67294390
  11. Yanushevich O.O., Tsarev V.N., Nikolaeva E.N., Balmasova I.P., Ippolitov E.V., Tsareva T.V., Podporin M.S., Ponomareva A.G. The First domestic experience of detecting the association of anaerobic bacteria Filifactor alocis and Porphyromonas gingivalis by molecular biological methods in periodontal diseases and comorbid pathology (comparative research). Annals of the Russian Academy of Medical Sciences. 2022; 6: 437—446 (In Russian). eLIBRARY ID: 50359379
  12. Nikolaeva E.N., Yanuchevich O.O., Tsareva T.V., Podporin M.S., Tsarev V.N. Filifactor alocis and its associations with anaerobic bacteria in patients with periodontitis and cardiovascular diseases. Clinical Dentistry (Russia). 2023; 4: 71—79 (In Russian). eLIBRARY ID: 59397994
  13. Al-hebshi N.N., Al-Alimi A., Taiyeb-Ali T., Jaafar N. Quantitative analysis of classical and new putative periodontal pathogens in subgingival biofilm: a case-control study. J Periodontal Res. 2015; 50 (3): 320—9. PMID: 25040261
  14. Rebrikov D.V. Real-time PCR. Moscow: Knowledge Lab, 2024. Pp. 109—140 (In Russian).
  15. Samusenkov V.O., Yumashev A.V., Vlasova N.N., Borisov V.V., Tsarev V.N., Ippolitov E.V., Podporin M.S., Tsareva T.V. Substantiation of use of photodynamic therapy in experimental research in vitro with strains of periodontopathogenic bacteria and fungi Candida. Journal of Global Pharma Technology. 2020; 2: 178—186. https://tinyurl.com/jgpt3250
  16. Suresh Unniachan A., Krishnavilasom Jayakumari N., Sethuraman S. Association between Candida species and periodontal disease: A systematic review. Curr Med Mycol. 2020; 6 (2): 63—68. PMID: 33628985
  17. Slazhneva E., Tikhomirova E., Tsarev V., Orekhova L., Loboda E., Atrushkevich V. Candida species detection in patients with chronic periodontitis: A systematic review and meta-analysis. Clin Exp Dent Res. 2022; 8 (6): 1354—1375. PMID: 35903878

Downloads

Received

August 15, 2025

Accepted

October 13, 2025

Published on

December 18, 2025