Experimental study of regenerative processes in the oral mucosa in response to various injuries using herbal extracts in gels

Abstract

The regeneration of the rat oral mucosa was studied after inflicting injuries with a scalpel, a mechanical drill, and an electrocoagulator. The wounds were treated with gels containing extracts of chamomile flowers (Group I), Bupleurum herb (Group II), and based on chamazulene (Group III). The assessment of regeneration was performed by studying histological specimens on the 4th and 7th days after injury. The histomorphometric evaluation of soft tissue regeneration parameters in the foci of various types of damage included semi-quantitative methods (angiogenesis and epitheliogenesis) along with a quantitative method for objectifying the nature of the inflammatory infiltrate. The best regeneration results were obtained in Group III for all types of simulated injuries, both on day 4 and on day 7. In the other animal groups, the severity of inflammatory changes was significantly higher than in Group III. The regenerative process in the control group with a lacerated wound significantly prevailed over Groups I and II in cases of incised wounds and electrocoagulation wounds.
Conclusions.
The obtained data show a significantly higher rate of epitheliogenesis and angiogenesis in the regeneration focus after using chamazulene, which indirectly indicates a more favorable effect of this preparation on the regeneration processes of the mucosa, regardless of the type of simulated injury.