DOI:

10.37988/1811-153X_2025_2_154

Experimental substantiation of in vitro anti-radical and immunomodulating properties of injection form of drugs containing hyaluronic acid

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Authors

  • Yu.L. Vasil’ev 1, 2, Doctor of Science in Medicine, full professor of the Operative surgery and topographic anatomy Department; senior researcher at the Antimicrobial Photodynamic Therapy Lab
    ORCID: 0000-0003-3541-6068
  • I.N. Usmanova 3, Doctor of Science in Medicine, full professor of the Therapeutic dentistry Department
  • I.A. Lakman 4, PhD in Engineering, assistant professor of the Biomedical engineering Department, head of the Scientific Lab for the study of socio-economic problems of regions
  • Yu.L. Bortsova 3, PhD in Biology, assistant professor of the Fundamental and applied microbiology Department
  • K.S. Mochalov 3, PhD in Biology, assistant professor of the Fundamental and applied microbiology Department
  • N.S. Shangareeva 5, 3rd year student at the Dental Faculty
    ORCID: 0009-0002-2901-8979
  • R.A. Khalitov 6, medical advisor
  • R. Sarmadian 1, 6th year student
    ORCID: 0009-0007-9612-7442
  • R.R. Akhmetvaleev 7, senior lecturer of the Digital technologies and modeling Department
    ORCID: 0000-0002-7526-353X
  • 1 Sechenov University, 119048, Moscow, Russia
  • 2 MIREA — Russian Technological University, 125993, Moscow, Russia
  • 3 Bashkir State Medical University, 450000, Ufa, Russia
  • 4 Ufa University of Science and Technology, 450076, Ufa, Russia
  • 5 RUDN University, 117198, Moscow, Russia
  • 6 “Axel-Pharma” LLC, 625001, Tyumen, Russia
  • 7 Ufa State Petroleum Technical University, 450064, Ufa, Russia

Abstract

An in vitro experimental study was conducted to evaluate the antiradical and immunomodulatory properties of injectable formulations containing 1% and 1.4% hyaluronic acid (HA) for the prevention and correction of conditions associated with excessive free radical activity, as well as the regulation of imbalances in oxidative-reduction processes and energy metabolism.
Materials and methods.
The experiment utilized model systems generating reactive oxygen species (ROS). To assess immunomodulatory effects, oxygen-dependent metabolism and ROS generation by phagocytes were simulated. Parameters of oxygen-dependent metabolism in blood were analyzed after adding 1% and 1.4% HA preparations, both in unstimulated and zymosan-stimulated conditions.
Results.
Both HA-containing formulations significantly reduced ROS generation, indicating their ability to neutralize free radicals and protect cells from oxidative stress. The antioxidant and antiradical properties of HA correlated with decreased ROS production, suggesting utility in mitigating oxidative damage. Additionally, the 1.0% and 1.4% HA preparations enhanced immune response efficiency by elevating basal and stimulated phagocyte activity and improving their functional reserve.
Conclusion.
The findings demonstrate the high efficacy of injectable hyaluronic acid formulations (1% and 1.4%) in preventing and correcting conditions linked to excessive free radical activity and imbalances in redox processes and energy metabolism.

Key words:

hyaluronic acid, phagocytic cells, neutrophils, oxidative processes, chemiluminescence, periodontal tissue

For Citation

[1]
Vasil’ev Yu.L., Usmanova I.N., Lakman I.A., Bortsova Yu.L., Mochalov K.S., Shangareeva N.S., Khalitov R.A., Sarmadian R., Akhmetvaleev R.R. Experimental substantiation of in vitro anti-radical and immunomodulating properties of injection form of drugs containing hyaluronic acid. Clinical Dentistry (Russia).  2025; 28 (2): 154—159. DOI: 10.37988/1811-153X_2025_2_154

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Received

May 31, 2025

Accepted

June 10, 2025

Published on

July 5, 2025