DOI:

10.37988/1811-153X_2023_3_125

Experimental microbiological justification of disinfection measures as a component of infectious safety in the practice of an orthopedic dentist

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Authors

  • V.N. Tsarev 1, PhD in Medical Sciences, full professor of the Microbiology, virology, immunology department, director of the Medico-dental research Institute
    ORCID ID: 0000-0002-3311-0367
  • A.N. Akavov 2, postgraduate at the Prosthodontics Department
    ORCID ID: 0009-0000-6987-6308
  • V.M. Karpova 1, PhD in Medical sciences, associate professor of the Dentistry diseases propaedeutics Department
    ORCID ID: 0000-0003-1003-6667
  • E.V. Tsareva 1, PhD in Medical Sciences, assistant professor of the Dentistry diseases propaedeutics Department
    ORCID ID: 0000-0001-8600-0447
  • A.A. Lastochkin 1, PhD in Medical Sciences, associate professor of the Microbiology, virology, immunology Department
    ORCID ID: 0009-0006-0473-4791
  • 1 Moscow State University of Medicine and Dentistry, 127473, Moscow, Russia
  • 2 Dagestan State Medical University, 367012, Makhachkala, Russia

Abstract

It is estimated that, on average, 6 to 9% of hospitalized patients suffer from the development and severe course of infectious complications caused by healthcare-associated pathogens each year. Solving the problems of infection safety, including in the practical work of a dentist, is an extremely urgent problem. The aim of the study was to experimentally substantiate the choice of preparations with antibiofilm action for optimizing the disinfection of dental impressions.
Materials and methods.
The microbiota of 34 silicone dental impressions was analyzed. Subsequently, the effect of preparations from the group of quaternary ammonium compounds (QAC) recommended for chemical disinfection (benzalkonium chloride, cetylpyridinium chloride, and benzyldimethyl[3-(myristoylamino)propyl]ammonium chloride) on the experimental biofilm was evaluated in an in vitro experiment using a scanning electron microscope.
Results.
The most significant level of microbial contamination exceeding the upper limit of known oral norms (104±102 CFU/mL) was registered for Streptococcus spp., S. sanguis, Corynebacterium spp., Fusobacterium spp., Enterococcus spp., Porphyromonas gingivalis (in the range of 105—107 CFU/mL). This made it possible to identify priority pathogens for the experiment of mixed biofilm formation and visual control of its destruction using QAC. The proposed experimental models can be used to select the optimal antiseptic in the range of 0.05—0.1%. Complete eradication of experimental biofilm pathogens and planktonic forms occurred when 0.1% solutions of biocidal agents were applied.
Conclusion.
QAC causes destruction of experimental biofilm. Miramistin at a concentration of 0.05% showed more pronounced activity against the strains of anaerobic microbiota studied.

Key words:

infection complication, disinfection by immersion, dental impressions, quaternary ammonium derivatives, miramistin

For Citation

[1]
Tsarev V.N., Akavov A.N., Karpova V.M., Tsareva E.V., Lastochkin A.A. Experimental microbiological justification of disinfection measures as a component of infectious safety in the practice of an orthopedic dentist. Clinical Dentistry (Russia).  2023; 26 (3): 125—133. DOI: 10.37988/1811-153X_2023_3_125

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Received

July 27, 2023

Accepted

August 7, 2023

Published on

September 24, 2023