The state of microbiocenosis in patients with bullous lesions of the oral mucosa according to the PCR diagnostic method

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Authors

  • O.F. Rabinovich 1, PhD in Medical Sciences, head of the Oral mucosa diseases Department
    ORCID ID: 0000-0002-1018-9017
  • I.M. Rabinovich 1, PhD in Medical Sciences, full professor of the Therapeutic dentistry Division and the Caries and endodontics Department
    ORCID ID: 0000-0001-8539-814X
  • E.S. Abramova 1, PhD in Medical Sciences, senior researcher of the Oral mucosa diseases Group
  • K.V. Umarova 1, PhD in Medical Sciences, researcher of the Oral mucosa diseases Group
    ORCID ID: 0000-0003-3985-8058
  • 1 Central research institute of dental and maxillofacial surgery, 119021, Moscow, Russia

Abstract

Autoimmune bullous lesions are a group of inflammatory diseases affecting the skin and mucous membranes, with a severe chronic course. Microorganisms that colonize the skin and mucous membranes and make up the microbiome can influence the course of inflammatory diseases. The purpose of this study was to investigate the oral mucosal microbiome in patients with bullous lesions by polymerase chain reaction (PCR).
Materials and methods.
We studied scrapings from the surface of lesions of the oral mucosa in 25 patients with pemphigus vulgaris, 35 patients with pemphigoid bullosa and 40 people with bullous form of ruber lichen planus. Fusobacterium, Str. pneumoniae, Candida albicans, Ureaplasma, and 3 types of viruses: human papilloma virus 16, Epstein-Barr virus, and cytomegalovirus were determined.
Results.
Str. pneumoniae was detected most frequently in patients with bullous lesions: in 42.5% of cases in patients with pemphigus vulgaris, in 58.3% of cases in patients with pemphigoid bullosa and in 68.8% of cases in patients with bullous form of ruber lichen planus. Candida albicans was second most frequently detected: in 33.0% of patients with pemphigoid bullosa, in 28.6% of patients with pemphigus vulgaris, and in 25.0% of patients with bullous form of ruber lichen planus. Among viruses, Epstein—Barr virus was most frequently detected in patients with bullous lesions of oral mucosa (in 66.7, 75, and 50% of patients with pemphigus vulgaris, pemphigoid bullosa, and bullous form of ruber lichen planus, respectively). However, cytomegalovirus was practically undetectable in scrapes in patients with pemphigoid bullosa and ruber lichen planus. The frequency of detection of associations of bacteria, fungi and viruses in scrapes in patients with pemphigoid bullosa reached 38.5%.
Conclusions.
The obtained data indicate a pronounced violation of oral microbiocenosis and microbial dysbiosis of the oral mucosa in patients with bullous lesions.

Key words:

oral mucosa, bullous lesions, microbiocenosis, microbiota, microbiome, polymerase chain reaction

For Citation

[1]
Rabinovich O.F., Rabinovich I.M., Abramova E.S., Umarova K.V. The state of microbiocenosis in patients with bullous lesions of the oral mucosa according to the PCR diagnostic method. Clinical Dentistry (Russia).  2021; 24 (3): 47—51

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Received

July 2, 2021

Accepted

September 4, 2021

Published on

September 1, 2021